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Item 1 of 7
HHLA2 Antibody (N-term)
Catalog Number: orb1938823
| Catalog Number | orb1938823 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids of human HHLA2. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q9UM44 |
| MW | 46850 Da |
| Tested applications | FC, IHC-P, WB |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:2000, WB: 1:2000, IHC-P: 1:50~100, IHC-P: 1:25, IHC-P: 1:25 |
| Antibody Type | Primary Antibody |
| Clone Number | RB30079 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | HERV-H LTR-associating protein 2, Human endogenous Read more... |
| Note | For research use only |
| NCBI | NP_009003.1 |
All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: Human lung lysate. Lane 3: Human pancreas lysate. Lane 4: Human testis lysate. Lane 5: MDA-MB-453 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-HHLA2 Antibody (N-term) at 1:2000 dilution. Lane 1: Human kidney lysate. Lane 2: Human lung lysate. Lane 3: Human testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HHLA2 Antibody (N-term) at 1:2000 dilution + Human kidney tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 70 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
HHLA2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHLA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Staining HHLA2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.
Staining HHLA2 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.
