ERBB2 Antibody

SKU: orb1253801

Description

ERBB2 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: HER2 (Phospho-Tyr877) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y (p) -H-A) derived from Human HER2.
• Target: ERBB2
• Molecular Weight: 185 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, BHLHE39, C-erbB-2, ErbB2

Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using HER2 (Phospho-Tyr877).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr877).

Quick Database Links

Gene Symbol

ERBB2

UniProt

P04626

RefSeq (Protein):NP_001005862.1