HER2 Antibody

SKU: orb385830

Description

HER2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. [RefSeq]

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-4ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER2 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 2KRI7
• Immunogen: Recombinant human protein was used as the immunogen for this HER2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of human MCF7 cells with HER2 antibody (clone 2KRI7, green) and Reddot nuclear stain (red).

Flow cytometry testing of human MCF7 cells with HER2 antibody (clone 2KRI7); Red = isotype control, Blue = HER2 antibody.