Hepatitis Virus A59 Nonstructural Protein 9 antibody

• Catalog Number: orb344441
• Category: Antibodies
• Description: Hepatitis Virus A59 Nonstructural Protein 9 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2C6.H1
• Tested applications: IF, WB
• Reactivity: Mouse
• Isotype: IgG2b
• Immunogen: This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
• Concentration: 2.01 mg/mL
• Dilution range: IF: 1:1,000, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antibody is directed against the MHV-A59 nsp9 protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.
• Conjugation: Unconjugated
• UniProt ID: P0C6V0
• NCBI: 25121567
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-Hepatitis Virus A59 Nonstructural Prote
• Note: For research use only
• Application notes: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of mouse L cells using Hepatitis Virus A59 Nonstructural Protein 9 antibody

Western blot analysis of various lysates Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Negative controls (lanes 2 and 4) show no staining using