Hemoglobin A (beta chain) antibody

• Catalog Number: orb420234
• Category: Antibodies
• Description: Hemoglobin A (beta chain) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 14G2.G11.F11
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
• Concentration: 1.04 mg/ml by UV absorbance at 280 nm
• Dilution range: ELISA: 1:20,000, WB: 1ug/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform. Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbS, HbF (gamma), or HbC forms. HbA antibody cross reacts with HbA-2.
• Conjugation: Unconjugated
• UniProt ID: P68871
• NCBI: NP_000509.1
• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-HbA antibody, mouse anti-hemoglobin ant
• Note: For research use only
• Application notes: Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA, SDS-Page, and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
• Expiration Date: 12 months from date of receipt.

Western Blot of Mouse Anti-human hemoglobin (HbA) Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Human HbA (0.1µg) [+]. Lane 3: Human HbS (0.1µg) [-]. Lane 4: Human Heart Whole Cell Lysate (2.0µg) [+]. Lane 5: Human H

Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. L

Lateral Flow Results of Anti-HbA (Hemoglobin A) and Anti-HbS (Hemoglobin beta S) Antibodies