Hemoglobin A (beta chain) Antibody

SKU: orb420234

Description

Hemoglobin A (beta chain) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:20,000, WB: 1ug/mL
• Reactivity: Human
• Application Notes:
  Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA, SDS-Page, and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: 14G2.G11.F11
• Immunogen: Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform. Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbS, HbF (gamma), or HbC forms. HbA antibody cross reacts with HbA-2.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01% (w/v) Sodium Azide
• Concentration: 1.04 mg/ml by UV absorbance at 280 nm
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-HbA antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit, Hemoglobin subunit beta, Hemoglobin beta chain, HBB, HBA, Hbβ Antibody, LVV-hemorphin-7, Spinorphin, Sickle Cell Disease (SCD)

Lateral Flow Results of Anti-HbA (Hemoglobin A) and Anti-HbS (Hemoglobin beta S) Antibodies. Triplicate test strips are spotted with a control, Anti-HbA antibody (p/n orb420234) 0.5 µl at 250 ug/ml, and Anti-HbS antibody 0.5 µl at 1 mg/ml. Recombinant HbA (left group) or recombinant HbS (right group) are observed to react with the corresponding antibodies specifically, leading to blue dots.

Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50 ng per lane. Primary antibody: Anti-HbA antibody at 1 µg/ml overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348644 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 2 specific to HbA and cross reactivity to HbA-2 seen in Lane 3.

Western Blot of Mouse Anti-human hemoglobin (HbA) Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Human HbA (0.1 µg) [+]. Lane 3: Human HbS (0.1 µg) [-]. Lane 4: Human Heart Whole Cell Lysate (2.0 µg) [+]. Lane 5: Human HeLa Whole Cell Lysate (10.0 µg) [-]. Primary Antibody: Anti-HbA at 1.0 µg/ml overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Peroxidase Conjugated (p/n orb347506) at 1:40000 at RT for 30 mins. Block: BlockOut Buffer (p/n orb348644). Predicted MW: ~16kDa. Observed MW: ~11kDa monomer, ~25kDa dimer. Exposure: 30 sec.

Quick Database Links

UniProt

P68871

RefSeq (Protein):NP_000509.1