HBB Antibody (C-term)

• Catalog Number: orb1938112
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18724
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 15998 Da
• Target: This HBB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-107 amino acids from the C-terminal region of human HBB.
• UniProt ID: P68871
• NCBI: NP_000509.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Hemoglobin subunit beta, Beta-globin, Hemoglobin b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-HBB Antibody (C-term) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: human liver lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 16 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

HBB Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of HBB Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HBB Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Overlay histogram showing K562 cells (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.