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NDC80 Antibody

SKU: orb1247402

Description

NDC80 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75-80kDa band observed in Hela lysate (predicted MW of 74kDa according to NP_006092). Recommended for use at 0.5-2µg/mL.Immunohistochemistry:Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-YEKKATLIKSSEE
TargetNDC80
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NDC80, HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1, KNTC2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3, hsNDC80, kinetochore associated 2

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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