Hec1 rabbit pAb

• Catalog Number: orb767343
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Hec1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400
• MW: 73
• Tested applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-NDC80 antibody, anti-HEC antibody, anti-HEC1
• Note: For research use only

Immunofluorescence analysis of Hela cell. 1, Hec1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Kif 7 Monoclonal Antibody (3F8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of Jurkat cells using Hec1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HUVEC cells, using KNTC2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, using KNTC2 Antibody. The lane on the right is blocked with the synthesized peptide.