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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IF/ICC: 1/200 - 1/1000; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | B9I8E7 |
| Immunogen | Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli. |
| Target | FDA approved drug target related |
| Molecular Weight | 48.8kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HD3; RPD3; RPD3-2
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Western blot analysis of Hela (Lane1), NIH/3T3 (Lane2), C6 (Lane3) and COS (Lane4) cell lysate using HDAC3 antibody
Confocal immunofluorescence analysis of Hela cells using HDAC3 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
HDAC3 Antibody (orb97554)
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