HDAC3 Antibody

• Catalog Number: orb1260861
• Category: Antibodies
• Description: HDAC3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human HDAC3 (NP_003874.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: HDAC3
• UniProt ID: O15379
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HDAC3, RPD3, SMAP45, HD3, Histone deacetylase 3, R
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Western blot analysis of extracts of mouse brain, using HDAC3 antibody (orb1260861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse liver using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HDAC3 antibody (orb1260861) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using HDAC3 Polyclonal Antibody (orb1260861) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.