HCN4 Antibody

SKU: orb67410

Description

Mouse Anti-Rat HCN4 Monoclonal IgG1

Research Area

Cardiovascular Research, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: AM, FACS, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rabbit, Rat
• Application Notes:
  1 µg/ml of SMC-320 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N114/10 (Formerly sold as S114-10)
• Immunogen: Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4
• Target: HCN4
• Molecular Weight: 130kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Hyperpolarization activated cyclic nucleotide gated potassium channel 4, HCN 4, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10. Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at RT.

Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Quick Database Links

Gene Symbol

HCN4

UniProt

Q9JKA7

NCBI Gene ID

59266

RefSeq (Protein):NP_067690.1