HCN4 Rabbit Polyclonal Antibody

• Catalog Number: orb10785
• Category: Antibodies
• Description: HCN4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HCN4 (501-600/1203aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 129 kDa
• Target: HCN4
• UniProt ID: Q9Y3Q4
• RRID: AB_10751972
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HCN 4; Hyperpolarization activated cyclic nucleoti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-HCN4 antibody (orb10785), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-HCN4 (orb10785) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 129 kD, Observed band size: 129 kD.

Sample: Lane 1: Mouse Testis Lysates, Lane 2: Rat Testis Lysates, Lane 3: Human HL-60 cell Lysates, Lane 4: Human MCF-7 cell Lysates, Lane 5: Human A549 cell Lysates, Primary: Anti-HCN4 (orb10785) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 129kDa, Observed band size: 129kDa.