HA EPITOPE TAG antibody

• Catalog Number: orb345398
• Category: Antibodies
• Description: HA EPITOPE TAG antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IHC, IP, WB
• Isotype: IgG
• Immunogen: Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
• Concentration: 1.05 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:100,000, ChIP: User Optimized, IHC: 1:500 - 1:2,000, IP: User Optimized, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-HA epitope tag antibody, rabbit anti-h
• Note: For research use only
• Application notes: Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
• Expiration Date: 12 months from date of receipt.

References

Meese, Sandra et al. Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin Front Synaptic Neurosci, 9, 13 (2017)

PubMed: 29046633

Immunohistochemical staining of mouse endothelial cells using HA EPITOPE TAG antibody

Western blot analysis of Protein Marker Lysate using HA EPITOPE TAG antibody