HA Tag antibody

• Catalog Number: orb27986
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DYKDDDDK Tag is used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest is an amino-terminal or carboxy-terminal fusion. It is a useful tool for the localization and characterization of DDDDK tagged proteins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0411
• Tested applications: ChIP, IF, WB
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IF/ICC: 1:10-50, ChIP: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Target: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Alternative names: Anti-Tag from influenza hemagglutinin protein anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Simkin, Dina et al. Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo Cell Tissue Res, 371, 309-323 (2018)

PubMed: 29018970

Western blot analysis of protein bacterial lysate using HA Tag antibody

Confocal immunofluoroscence analysis of MRC-5 fibroblasts using HA Tag antibody

CHIP analysis of 35S:HATCL1 plants using HA Tag antibody

Confocal immunofluorescense analysis of endocytosis cell using HA tag antibody

Western blot analysis of HA tag antibody in HATCL1 plant

WB analysis of 12 tag protein using HA Tag antibody