HA Tag Antibody

SKU: orb27986

Description

Rabbit polyclonal antibody to DYKDDDDK Tag is used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest is an amino-terminal or carboxy-terminal fusion. It is a useful tool for the localization and characterization of DDDDK tagged proteins.

Images & Validation

• Tested Applications: ChIP, IF, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:10-50, ChIP: 1:100

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB0411
• Immunogen: Synthetic Peptide
• Target: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Tag from influenza hemagglutinin protein antibody

Quick Database Links

Gene Symbol

KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

Simkin, Dina et al. Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo Cell Tissue Res, 371, 309-323 (2018)

PubMed: 29018970