GULP1 Antibody

• Catalog Number: orb1247409
• Category: Antibodies
• Description: GULP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: EGTVFCLDPLDSRC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6ug/ml. An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration: 5-10 ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GULP1
• UniProt ID: Q9UBP9
• NCBI: NP_057399.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CED-6, GULP1, CED-6 protein, GULP, engulfment adap
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6ug/ml. An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration: 5-10 ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247409 staining (0.2 ug/ml) of Human Placenta lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1247409 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.