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Catalog Number | orb1258304 |
---|---|
Category | Antibodies |
Description | GSK3B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human GSK3B |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 46kDa |
Target | GSK3B |
UniProt ID | P49841 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta i Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of mouse brain, using GSK3B antibody (orb1258304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using GSK3B antibody (orb1258304) at dilution of 1:100. Blue: DAPI for nuclear staining.
IF, IH, IP, WB | |
Human, Mouse, Porcine, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Animal, Bovine, Canine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish | |
Canine, Equine, Goat, Guinea pig, Human, Mouse, Rat, Sheep, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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