GSK-3 Beta Mouse Monoclonal Antibody

SKU: orb500627

Description

GSK-3 Beta Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 3A6
• Immunogen: KLH conjugated synthetic peptide derived from human GSK-3 Beta
• Target: GSK3B
• Molecular Weight: 47 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3β; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Serine/threonine-protein kinase GSK3B; GSK3B_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of orb500627 3A6) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of orb500627 3A6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (orb500627) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (orb500627) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of orb500627 3A6) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (ascites of orb500627 3A6) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (orb500627) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Hela ko GSK-3 Beta (Human) Cell Lysate at 30 ug, Primary: Anti-GSK-3 Beta (orb500627) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Pancreas (Mouse) Lysate at 40 ug, Lane 3: Muscle (Rat) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Hela (Human) Cell Lysate at 30 ug, Lane 6: MCF-7 (Human) Cell Lysate at 30 ug, Lane 7: A431 (Human) Cell Lysate at 30 ug, Lane 8: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-GSK-3 Beta (orb500627) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

GSK3B

UniProt

P49841