GRIK3 Antibody

• Catalog Number: orb1246539
• Category: Antibodies
• Description: GRIK3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Human
• Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-KIRQLPIDSDDSRP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GRIK3
• UniProt ID: Q13003
• NCBI: NP_000822.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRIK3, GLUR7, glutamate receptor, ionotropic, kain
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.

orb1246539 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.