GRIK3 Antibody

SKU: orb1246539

Description

GRIK3 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1µg/mL. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KIRQLPIDSDDSRP
• Target: GRIK3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRIK3, GLUR7, glutamate receptor, ionotropic, kainate 3, EAA5, GLR7, GluR7a, dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)), excitatory amino acid receptor 5, glutamate receptor 7

orb1246539 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GRIK3

UniProt

Q13003

RefSeq (Protein):NP_000822.2