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GRIK3/GLUR7 Antibody

SKU: orb19926

Description

Goat polyclonal antibody to GRIK3

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:64000, WB: 0.3-1 μg/ml
ReactivityMouse, Rat
Predicted ReactivityBovine, Canine, Human
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetGRIK3 / GLUR7
Protein SequenceKIRQLPIDSDDSRP
Molecular Weight104
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GRIK3 antibody, anti GLUR7 antibody, anti glutamate receptor, ionotropic, kainate 3 antibody, anti EAA5 antibody, anti GLR7 antibody, anti GluR7a antibody, anti dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)) antibody, anti excitatory amino acid receptor 5 antibody, anti glutamate receptor 7 antibody

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GRIK3/GLUR7 Antibody

orb19926 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GRIK3/GLUR7 Antibody (orb19926)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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