GRIA4 Antibody

• Catalog Number: orb1246660
• Category: Antibodies
• Description: GRIA4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KKLDQREYPGSETP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx.100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2kDa according to NP_001070711.2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GRIA4
• UniProt ID: P48058
• NCBI: NP_001070712.1, NP_001070711.2, NP_000820.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRIA4 , glutamate receptor, ionotrophic, AMPA 4 ,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx.100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2kDa according to NP_001070711.2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246660 (2 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246660 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.