GRIA4 Antibody

SKU: orb20006

Description

Goat polyclonal antibody to GRIA4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 5-10µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.WB: Approx. 90 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kDa according to NP_001070711.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GRIA4
• Protein Sequence: KKLDQREYPGSETP
• Molecular Weight: 101; 99.2; 49.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GRIA4 antibody, anti glutamate receptor, ionotrophic, AMPA 4 antibody, anti GLUR4 antibody, anti GLUR4C antibody, anti GLURD antibody, anti AMPA-selective glutamate receptor 4 antibody

orb20006 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20006 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GRIA4

NCBI Gene ID

2893