Granzyme B Antibody

• Catalog Number: orb639513
• Category: Antibodies
• Description: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GZMB/2403
• Tested applications: ELISA
• Reactivity: Human
• Isotype: Mouse IgG2b, lambda
• Immunogen: A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.
• Dilution range: ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Granzyme B antibody is available for research use only.
• UniProt ID: P10144
• Storage: Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Granzyme B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Granzyme B antibody (clone GZMB/2403). These results demonstrate the foremost specificity of the GZMB/2403 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Granzyme B antibody (clone GZMB/2403) as confirmation of integrity and purity.