Granzyme B Antibody

• Catalog Number: orb1519946
• Category: Antibodies
• Description: Granzyme B Antibody
• Target: Granzyme B
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 50 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Purity: The monoclonal antibody was purified from cell culture supernatant
• Immunogen: Residues 19-247 (mature protein)
• UniProt ID: P10144
• Tested applications: ICC, IF, IHC, WB
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Antibody Type: Primary Antibody
• Clone Number: BLR022E
• Storage: 2 - 8°C
• Alternative names: C11; cathepsin G-like 1; CCPI; CGL1; CGL-1; CSPB;
• Background: Granzyme B is a member of the granzyme subfamily o
• Note: For research use only
• NCBI: NP_004122.2
• Expiration Date: 12 months from date of receipt.

Detection of human Granzyme B by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody

Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human Granzyme B by immunocytochemistry. Sample: FFPE section of Granzyme B over-expressing cells (left) and Granzyme H over-expressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human Granzyme B (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: Opal™. Counterstain: DAPI (blue)

Detection of human Granzyme B (shaded) in SR cells by flow cytometry. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG