GPSM2 Antibody

• Catalog Number: orb1247425
• Category: Antibodies
• Description: GPSM2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NRLKGKKYKTNSSTK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03ug/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GPSM2
• UniProt ID: P81274
• NCBI: NP_037428.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GPSM2, G-protein signalling modulator 2 (AGS3-like
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03ug/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247425 (0.01 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247425 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.