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GPSM2 Antibody

SKU: orb1247425

Description

GPSM2 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) and Human Liver lysates (calculated MW of 75.8kDa according to NP_037428.2). Recommended concentration: 0.01-0.03ug/ml. An additional band of unknown identity was also consistently observed at 26-28kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NRLKGKKYKTNSSTK
TargetGPSM2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

GPSM2, G-protein signalling modulator 2 (AGS3-like, C. elegans) , HGNC:29501, LGN, Pins, LGN protein

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GPSM2 Antibody

orb1247425 (0.01 ug/ml) staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GPSM2 Antibody

orb1247425 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GPSM2 Antibody (orb1247425)

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0.1 mg
$ 920.00
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