GPM6A Rabbit Polyclonal Antibody

• Catalog Number: orb183993
• Category: Antibodies
• Description: GPM6A Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GPM6A (151-250/278aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: GPM6A
• UniProt ID: P51674
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GPM6A; GPM6A_HUMAN; M6a; M6 a; M6-a; Neuronal memb
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-GPM6A (orb183993) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: Lane 1: Spinal cord (Rat) Lysate at 40 ug, Lane 2: Lung (Rat) Lysate at 40 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Lane 4: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-GPM6A (orb183993) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 28 kD.