GOT1 Antibody

SKU: orb1247431

Description

GOT1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87(9):3484-8; PMID: 2185471). Recommended concentration: 0.01-1µg/mL.Immunohistochemistry:In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration: 2-3µg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RSYRYWDAEKR
• Target: GOT1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GOT1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) , GIG18, aspartate aminotransferase 1, growth-inhibiting protein 18

orb1247431 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247431 (2.5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247431 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GOT1

UniProt

P17174

RefSeq (Protein):NP_002070.1