GOT1 (aa 157-167) Antibody

SKU: orb19708

Description

Goat polyclonal to GOT1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 0.01-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration, 2-3ug/ml.WB: Approx 38-40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87(9):3484-8; PMID: 2185471). Recommended concentration: 0.01-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GOT1 (aa 157-167)
• Protein Sequence: RSYRYWDAEKR
• Molecular Weight: 46.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GOT1 antibody, anti glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) antibody, anti GIG18 antibody, anti aspartate aminotransferase 1 antibody, anti growth-inhibiting protein 18 antibody

Western blot analysis of Human Liver lysate using GOT1 antibody

Immunohistochemical staining of Human Heart using GOT1 antibody

Immunohistochemical staining of Human Kidney using GOT1 antibody

Quick Database Links

Gene Symbol

GOT1 (aa 157-167)

NCBI Gene ID

2805