Goat IgG (H&L) Secondary Antibody Fluorescein Conjugated

• Catalog Number: orb346985
• Category: Antibodies
• Description: Goat IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Goat
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.
• Immunogen: Goat IgG whole molecule
• Tested applications: DOT, FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Goat IgG Fluorescein Antibody has been tested by dot blot and western blot. Secondary antibody reagents are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods
• Antibody Type: Secondary Antibody
• Storage: Store anti-goat FITC conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: FITC Conjugated Secondary Antibody, Anti-Goat FITC
• Note: For research use only

Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated. Lane 1: Goat IgG. Load: 50 ng per lane. Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.