ZYX Antibody

• Catalog Number: orb20416
• Category: Antibodies
• Description: Goat polyclonal antibody to ZYX
• Target: ZYX
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QQREKPRVQEKQH
• RRID: AB_10750600
• MW: 61.3
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kDa according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ZYX antibody, anti zyxin antibody, anti ESP-2
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 7791
• Expiration Date: 12 months from date of receipt.

orb20416 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.