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ZYX Antibody

SKU: orb20416

Description

Goat polyclonal antibody to ZYX

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:4000, WB: 0.1-0.3 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kDa according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetZYX
Molecular Weight61.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti ZYX antibody, anti zyxin antibody, anti ESP-2 antibody, anti HED-2 antibody

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ZYX Antibody

orb20416 (0.1ug/ml) staining of Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ZYX Antibody (orb20416)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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