VPS35/MEM3 Antibody

SKU: orb18631

Description

Goat polyclonal antibody to VPS35

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 1.0-3.0 μg/ml, IHC-P: 5-8 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.WB: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3 μg/ml.Experiment Notes: Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please see Scott KL et al.; Nature. 2009 Jun 25;459(7250):1085-90.; PMID: 19553991.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: VPS35 / MEM3
• Molecular Weight: 91.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration:

  1-3 μg/ml

• Disclaimer: For research use only

Alternative Names

anti VPS35 antibody, anti MEM3 antibody, anti FLJ10752 antibody, anti FLJ13588 antibody, anti FLJ20388 antibody, anti DKFZp434E1211 antibody, anti DKFZp434P1672 antibody, anti vacuolar protein sorting 35 (yeast) antibody, anti maternal-embryonic 3 antibody, anti vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, anti vacuolar protein sorting 35 antibody

Western blot analysis of Human (A) Mouse (B) and Rat (C) Brain lysate using VPS35 antibody.

Immunohistochemical staining of Human Prostate using VPS35 antibody

Immunohistochemical staining of Human Prostate using VPS35 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using VPS35 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells using VPS35 antibody.

Western blot analysis of HepG2 (A) and HEK293 (B) cell lysate using VPS35 antibody.

Quick Database Links

Gene Symbol

VPS35 / MEM3

NCBI Gene ID

55737