VPS35 Antibody

• Catalog Number: orb1246790
• Category: Antibodies
• Description: VPS35 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SPESEGPIYEGLIL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in Human Brain (Cerebellum), Human Heart and Human Ovary and Mouse Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). Recommended concentration: 1.0-3.0ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OScells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VPS35
• UniProt ID: Q96QK1
• NCBI: NP_060676.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp43
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in Human Brain (Cerebellum), Human Heart and Human Ovary and Mouse Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). Recommended concentration: 1.0-3.0ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OScells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246790 (1 ug/ml) staining of Human Cerebellum (A) and Mouse Brain (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246790 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246790 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246790 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.