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Catalog Number | orb20445 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to UNC45B |
Target | UNC45B |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Canine, Mouse, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | RSDKLRQKIFKER |
RRID | AB_10750230 |
MW | 104, 104 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:2000, WB: 0.3-1 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 90kDa band observed in Human Skeletal Muscle lysates (calculated MW of 104kDa according to NP_775259.1). Recommended concentration: 0.3-1 μg/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti unc-45 homolog B (C. elegans) antibody, anti Read more... |
Note | For research use only |
Entrez | 146862 |
orb20445 (0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |