Goat anti-UBR2 Antibody

• Catalog Number: orb20366
• Category: Antibodies
• Description: Goat polyclonal antibody to UBR2
• Target: UBR2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SCPKSGGDKSRAPT
• RRID: AB_10751309
• MW: 201
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.01 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 150kDa and 100kDa in Human Lung lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 201kDa according to NP_056070.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti UBR2 antibody, anti ubiquitin protein ligase
• Note: For research use only
• Entrez: 23304

Immunohistochemical staining of Human Cortex using UBR2 antibody