TXNDC4 Antibody

SKU: orb19872

Description

Goat polyclonal to TXNDC4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.2-0.6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 47kDa band observed in lysates of cell line K562 (calculated MW of 47.0kDa according to NP_055866.1). Recommended concentration: 0.2-0.6 μg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TXNDC4
• Protein Sequence: EQKDSDNYRVFER
• Molecular Weight: 47
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TXNDC4 antibody, anti thioredoxin domain containing 4 (endoplasmic reticulum) antibody, anti ERP44 antibody, anti KIAA0573 antibody, anti OTTHUMP00000063799 antibody, anti endoplasmic reticulum resident protein 44 kDa antibody

orb19872 (0.2ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

TXNDC4

NCBI Gene ID

23071