TRPC6 Antibody

SKU: orb19085

Description

Goat polyclonal antibody to TRPC6

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:2000, WB: 0.3-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 80kDa band in Human Spleen and Testis lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106kDa according to NP_004612.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedExperiment Notes: FACS: This antibody has been proven to specifically react to TRPC6 in FACS.

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Key Properties

• Clonality: Polyclonal
• Target: TRPC6
• Protein Sequence: EKLSMEPNQEETN
• Molecular Weight: 106
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TRPC6 antibody, anti transient receptor potential cation channel, subfamily C, member 6 antibody, anti HGNC:12338 antibody, anti TRP6 antibody, anti transient receptor potential channel 6 antibody, anti FLJ11098 antibody, anti FLJ14863 antibody, anti FSGS2 antibody, anti TRP6 antibody

Quick Database Links

Gene Symbol

TRPC6

NCBI Gene ID

7225