Goat anti-TREM2 Antibody

SKU: orb19542

Description

Goat polyclonal antibody to TREM2

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution Range: ELISA: 1:128000, IHC-P: 2-4 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows staining of macrophages. Recommended concentration, 2-3ug/ml.WB: Preliminary experiments gave bands at approx 80kDa and 30kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 25.4kDa according to NP_061838.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TREM2
• Protein Sequence: HGQKPGTHPPSELD
• Molecular Weight: 25.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TREM2 antibody, anti triggering receptor expressed on myeloid cells 2 antibody, anti TREM-2 antibody, anti Trem2a antibody, anti Trem2b antibody, anti Trem2c antibody, anti OTTHUMP00000039771 antibody, anti triggering receptor expressed on myeloid cells 2a antibody

Immunohistochemical staining of Human Spleen using TREM2 antibody

Western blot of Human Cerebellum using TREM2 antibody

Quick Database Links

Gene Symbol

TREM2

NCBI Gene ID

54209