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Goat anti-Trem2 (mouse) Antibody

Catalog Number: orb20251

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb20251
CategoryAntibodies
DescriptionGoat polyclonal antibody to Trem2
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IF
ReactivityMouse
Dilution rangeELISA: 1:32000, WB: 1 μg/ml, IHC-P: 10 μg/ml
ConjugationUnconjugated
MW24.5
TargetTrem2 (mouse)
Protein SequenceQVEHSTSRNQET
RRIDAB_10919900
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti Trem2 antibody, anti triggering receptor expr
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 28kDa band observed in Mouse Liver lysates (calculated MW of 24.5kDa according to NP_112544.1). Recommended concentration: 0.3-1 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-Trem2 (mouse) Antibody

orb20251 Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Goat anti-Trem2 (mouse) Antibody

orb20251 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Goat anti-Trem2 (mouse) Antibody

orb20251 (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.