Goat anti-Trem2 (mouse) Antibody

SKU: orb20251

Description

Goat polyclonal antibody to Trem2

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 28kDa band observed in Mouse Liver lysates (calculated MW of 24.5kDa according to NP_112544.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Trem2 (mouse)
• Protein Sequence: QVEHSTSRNQET
• Molecular Weight: 24.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Trem2 antibody, anti triggering receptor expressed on myeloid cells 2 antibody, anti Trem2a antibody, anti Trem2b antibody, anti Trem2c antibody, anti triggering receptor expressed on myeloid cells 2a antibody, anti triggering receptor expressed on myeloid cells 2b antibody, anti triggering receptor expressed on myeloid cells 2c antibody

orb20251 Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb20251 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb20251 (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Trem2 (mouse)