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Catalog Number | orb1539139 |
---|---|
Category | Antibodies |
Description | RNF139/TRC8 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Human, Monkey |
Immunogen | Peptide with sequence C-HTGAAAEEFNDDT, from the C-Terminus of protein sequence according to NP_009149.2. |
Concentration | 0.5 mg/ml |
Dilution range | IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.3 - 1 µg/ml) |
Conjugation | Unconjugated |
Target | RNF139 / TRC8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
Alternative names | RNF139, HRCA1, RCA1, TRC8, Ring finger protein 139 Read more... |
Note | For research use only |
Application notes | Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx. 150kDa and 50kDa in Human Skeletal Mucle lysate after 0.5 ug/ml antibody staining. This protein has a calculated MW of 76.0kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 ug/ml. |
Expiration Date | 12 months from date of receipt. |
Goat Anti-TRC8 Antibody (1 µg/ml) staining of Human Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.