TIF1A/TRIM24 Antibody

SKU: orb18826

Description

Goat polyclonal antibody to TRIM24

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 1ug/ml, IHC-P: 4-6µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1 μg/ml, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some of the bands we observe given the predicted sizes of approx. 117kDa and 121kDa according to NP_003843 and NP_056989. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: TIF1A / TRIM24
• Protein Sequence: KRLKSIEERQLLK
• Molecular Weight: 117, 113
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TRIM24 antibody, anti TIF1 antibody, anti transcriptional intermediary factor 1 antibody, anti PTC6 antibody, anti TF1A antibody, anti TIF1A antibody, anti Tif1a antibody, anti hTIF1 antibody, anti TIF1ALPHA antibody, anti transcriptional intermediary factor 1 alpha antibody, anti tripartite motif protein 24 antibody, anti tripartite motif-containing 24 antibody, anti RNF82 antibody

Immunohistochemical staining of Human Breast cancer using TRIM24 antibody

Quick Database Links

Gene Symbol

TIF1A / TRIM24

NCBI Gene ID

8805