TIF1 alpha Antibody / TIF1A / TRIM24

SKU: orb2636810

Description

TIF1 alpha, also referred to as TIF1A and TRIM24, is a transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. [UniProt]

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the TIF1 alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-TRIM24-1B12
• Immunogen: Recombinant full-length human Transcription intermediary factor 1-alpha protein was used as the immunogen for the TIF1 alpha antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed MCF7 cells using TIF1 alpha antibody (green, clone PCRP-TRIM24-1B12) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with TIF1 alpha antibody (blue, clone PCRP-TRIM24-1B12) and isotype control (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using TIF1 alpha antibody (clone PCRP-TRIM24-1B12). These results demonstrate the foremost specificity of the PCRP-TRIM24-1B12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

O15164