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| Catalog Number | orb18647 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to TCP1 |
| Target | TCP1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | SYEDAVHSGALND |
| RRID | AB_10754604 |
| MW | 60.3; 43.9 |
| Tested applications | ELISA, FC, IF, IHC, WB |
| Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 1-2 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration, 1-2ug/ml.WB: Approx 60kDa band observed in Hela, Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti TCP1 antibody, anti t-complex 1 antibody, ant Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 6950 |

Immunohistochemical staining of Human Testis using TCP1 antibody

Western blot analysis of Human Ovary (lane 1) lysate and HEK293 (lane 2) cell lysate using TCP1 antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using TCP1 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using TCP1 antibody.
ELISA, IHC, WB | |
Bovine, Canine, Porcine, Rat | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
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