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Catalog Number | orb20263 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CCT3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 60.5; 60.5; 56.4 |
Target | CCT3 / TCP1 |
Entrez | 7203 |
Protein Sequence | GHKKKGDDQSRQGG |
RRID | AB_10748960 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti CCT3 antibody, anti chaperonin containing TCP Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 65kDa band observed in Mouse and Rat Testis lysates (calculated MW of 60.5kDa according to Human NP_005989.3 and 60.6kDa according to Mouse NP_033966.1 and Rat NP_954522.1). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20263 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb20263 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.