CCT3/TCP1 Antibody

SKU: orb20263

Description

Goat polyclonal antibody to CCT3

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 65kDa band observed in Mouse and Rat Testis lysates (calculated MW of 60.5kDa according to Human NP_005989.3 and 60.6kDa according to Mouse NP_033966.1 and Rat NP_954522.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CCT3 / TCP1
• Molecular Weight: 60.5; 60.5; 56.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CCT3 antibody, anti chaperonin containing TCP1, subunit 3 (gamma) antibody, anti CCT-gamma antibody, anti CCTG antibody, anti PIG48 antibody, anti TCP-1-gamma antibody, anti TRIC5 antibody, anti OTTHUMP00000025735 antibody, anti T-complex protein 1, gamma subunit antibody, anti TCP1 (t-complex-1) ring complex, polypeptide 5 antibody, anti chaperonin containing TCP1, subunit 3 antibody

orb20263 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20263 (5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CCT3 / TCP1

NCBI Gene ID

7203