Synaptotagmin I Antibody

SKU: orb20167

Description

Goat polyclonal antibody to SYT1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2000, WB: 0.3-1 μg/ml, IHC-P: 4-6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Recommended concentration: 0.3-1 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: Synaptotagmin I
• Protein Sequence: RDLQSAEKEEQEK
• Molecular Weight: 47.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti synaptotagmin I antibody, anti DKFZp781D2042 antibody, anti SYT1 antibody, anti SYT antibody, anti synaptotagmin 1 antibody

Quick Database Links

Gene Symbol

Synaptotagmin I

NCBI Gene ID

6857