SOX2 Goat Polyclonal Antibody

SKU: orb443078

Description

SOX2 Goat Polyclonal Antibody recognizes the N-terminus of human SOX2. Validated for ELISA (1:32,000), WB (0.1-0.3 µg/ml), IHC (6-8 µg/ml), and flow cytometry (10 µg/ml). Reactivity includes human, with predicted reactivity for bovine, canine, and rat. For research use only.

Research Area

Stem Cell & Developmental Biology; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: P-ELISA: 1: 32000, WB: 0.1-0.3μg/ml, IHC: 6-8µg/ml, FC: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  WB: Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to )NP_003097.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/m. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

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Key Properties

• Immunogen: Peptide with the sequence YNMMETELKPPGPQQ-C, derived from the N-terminus of the protein sequence corresponding to NP_003097.1.
• Target: SOX2
• Molecular Weight: 34.3 kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SOX2; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2

Quick Database Links

Gene Symbol

SOX2

UniProt

P48431