SNX5 Antibody

SKU: orb18604

Description

Goat polyclonal antibody to SNX5

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration, 3-6ug/ml.WB: Approx. 48kDa band observed in lysates of Human Kidney and cell lines Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SNX5
• Protein Sequence: SLLQSCIDLFKNN
• Molecular Weight: 46.8; 34.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SNX5 antibody, anti FLJ10931 antibody, anti sorting nexin 5 antibody, anti RP11-504H3.2 antibody

orb18604 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18604 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

SNX5

NCBI Gene ID

27131