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Catalog Number | orb18604 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SNX5 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-6 μg/ml |
Conjugation | Unconjugated |
MW | 46.8; 34.9 |
Target | SNX5 |
Entrez | 27131 |
Protein Sequence | SLLQSCIDLFKNN |
RRID | AB_10748472 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti SNX5 antibody, anti FLJ10931 antibody, anti s Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration, 3-6ug/ml.WB: Approx. 48kDa band observed in lysates of Human Kidney and cell lines Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18604 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18604 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
ELISA, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |