SNX5 Antibody

• Catalog Number: orb18604
• Category: Antibodies
• Description: Goat polyclonal antibody to SNX5
• Target: SNX5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SLLQSCIDLFKNN
• RRID: AB_10748472
• MW: 46.8; 34.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration, 3-6ug/ml.WB: Approx. 48kDa band observed in lysates of Human Kidney and cell lines Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SNX5 antibody, anti FLJ10931 antibody, anti s
• Note: For research use only
• Entrez: 27131

orb18604 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18604 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.