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Item 1 of 7
Item 1 of 7
Goat anti-SIRT4 Antibody
Catalog Number: orb18632
| Catalog Number | orb18632 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to SIRT4 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Dilution range | ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-3 μg/ml |
| Conjugation | Unconjugated |
| MW | 35.2 |
| Target | SIRT4 |
| Entrez | 23409 |
| Protein Sequence | SRCGELLPLIDPC |
| RRID | AB_10750974 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti SIRT4 antibody, anti SIR2L4 antibody, anti si Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3ug/ml.WB: Approx. 38kDa band observed in Human Kidney and U937 lysates (calculated MW of 35.2kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb18632 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
orb18632 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb18632 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18632 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
