SIRT4 Antibody

• Catalog Number: orb18632
• Category: Antibodies
• Description: Goat polyclonal antibody to SIRT4
• Target: SIRT4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SRCGELLPLIDPC
• RRID: AB_10750974
• MW: 35.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3ug/ml.WB: Approx. 38kDa band observed in Human Kidney and U937 lysates (calculated MW of 35.2kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SIRT4 antibody, anti SIR2L4 antibody, anti si
• Research Area: Cancer, Cardiovascular Disease, DNA Damage
• Note: For research use only
• Entrez: 23409

orb18632 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb18632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb18632 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb18632 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.