Goat anti-Robo3 (mouse) Antibody

• Catalog Number: orb19560
• Category: Antibodies
• Description: Goat polyclonal antibody to Robo3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA
• Reactivity: Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.1 μg/ml
• Conjugation: Unconjugated
• MW: 143
• Target: Robo3 (mouse)
• Protein Sequence: RRQKQRKELSHYT
• RRID: AB_10746387
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Robo3 antibody, anti roundabout homolog 3 (Dr
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.