Robo3.2 (mouse aa1303-1316) Antibody

• Catalog Number: orb195325
• Category: Antibodies
• Description: Goat polyclonal antibody to Robo3
• Target: Robo3.2 (mouse aa1303-1316)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KESQGRGRGLEACR
• MW: 150
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:128000, WB: 1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa accordi. IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Robo3 antibody, anti roundabout homolog 3 (Dr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Cerebellum using Robo3 antibody.