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Catalog Number | orb195325 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Robo3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 150 |
Target | Robo3.2 (mouse aa1303-1316) |
Protein Sequence | KESQGRGRGLEACR |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti Robo3 antibody, anti roundabout homolog 3 (Dr Read more... |
Note | For research use only |
Application notes | WB: Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa accordi. IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Cerebellum using Robo3 antibody.