Restin/CLIP1 Antibody

SKU: orb20278

Description

Goat polyclonal antibody to CLIP1

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows structured cytoplasm staining in active epithelial cells. Recommended concentration, 2-4ug/ml.WB: Approx 160kDa band observed in Human Breast Cancer lysates (calculated MW of 161kDa according to NP_002947.1. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Restin / CLIP1
• Molecular Weight: 161; 157
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti CLIP1 antibody, anti CAP-GLY domain containing linker protein 1 antibody, anti CLIP antibody, anti CLIP-170 antibody, anti CLIP170 antibody, anti CYLN1 antibody, anti MGC131604 antibody, anti RSN antibody, anti Reed-Steinberg cell-expressed intermediate filament-associated protein antibody, anti cytoplasmic linker 1 antibody, anti restin antibody, anti restin (Reed-Steinberg cell-expressed intermediate filament-associated protein) antibody

orb20278 (1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20278 (2.5ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Prostate using CLIP1 antibody.

Quick Database Links

Gene Symbol

Restin / CLIP1

NCBI Gene ID

6249