CLIP1 Antibody (N-term)

SKU: orb1928190

Description

CLIP1 Antibody (N-term)

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Gallus, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 162246 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

CYLN1, RSN

CLIP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-CLIP1 Antibody (N-term) at 1:1000 dilution + A549 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 162 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CLIP1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLIP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-CLIP1 Antibody (N-term) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 162 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P30622